A RNA producing DNA hydrogel as a platform for a high performance RNA interference system

Jaejung Song, Minhyuk Lee, Taeyoung Kim, Jeongkyeong Na, Yebin Jung, Gyoo Yeol Jung, Sungjee Kim and Nokyoung Park ()
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Jaejung Song: Pohang University of Science and Technology (POSTECH)
Minhyuk Lee: Myongji University
Taeyoung Kim: Myongji University
Jeongkyeong Na: Department of Chemical Engineering, POSTECH
Yebin Jung: Department of Chemistry, POSTECH
Gyoo Yeol Jung: Pohang University of Science and Technology (POSTECH)
Sungjee Kim: Pohang University of Science and Technology (POSTECH)
Nokyoung Park: Myongji University

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract RNA interference (RNAi) is a mechanism in which small interfering RNA (siRNA) silences a target gene. Herein, we describe a DNA hydrogel capable of producing siRNA and interfering with protein expression. This RNAi-exhibiting gel (termed I-gel for interfering gel) consists of a plasmid carrying the gene transcribing siRNA against the target mRNA as part of the gel scaffold. The RNAi efficiency of the I-gel has been confirmed by green fluorescent protein (GFP) expression assay and RNA production quantification. The plasmid stability in the I-gel results in an 8-times higher transcription efficiency than that of the free plasmid. We further applied the I-gel to live cells and confirmed its effect in interfering with the GFP expression. The I-gel shows higher RNAi effect than plasmids in free form or complexed with Lipofectamine. This nanoscale hydrogel, which is able to produce RNA in a cell, provides a platform technology for efficient RNAi system.

Date: 2018
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DOI: 10.1038/s41467-018-06864-0

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