Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns

Nao Yamakawa, Jorick Vanbeselaere, Lan-Yi Chang, Shin-Yi Yu, Lucie Ducrocq, Anne Harduin-Lepers, Junichi Kurata, Kiyoko F. Aoki-Kinoshita, Chihiro Sato, Kay-Hooi Khoo, Ken Kitajima and Yann Guerardel ()
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Nao Yamakawa: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Jorick Vanbeselaere: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Lan-Yi Chang: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Shin-Yi Yu: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Lucie Ducrocq: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Anne Harduin-Lepers: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle
Junichi Kurata: Soka University, Hachioji
Kiyoko F. Aoki-Kinoshita: Soka University, Hachioji
Chihiro Sato: Nagoya University
Kay-Hooi Khoo: Academia Sinica
Ken Kitajima: Nagoya University
Yann Guerardel: Université de Lille, CNRS, UMR 8576 – UGSF—Unité de Glycobiologie Structurale et Fonctionnelle

Nature Communications, 2018, vol. 9, issue 1, 1-14

Abstract: Abstract The emergence of zebrafish Danio rerio as a versatile model organism provides the unique opportunity to monitor the functions of glycosylation throughout vertebrate embryogenesis, providing insights into human diseases caused by glycosylation defects. Using a combination of chemical modifications, enzymatic digestion and mass spectrometry analyses, we establish here the precise glycomic profiles of eight individual zebrafish organs and demonstrate that the protein glycosylation and glycosphingolipid expression patterns exhibits exquisite specificity. Concomitant expression screening of a wide array of enzymes involved in the synthesis and transfer of sialic acids shows that the presence of organ-specific sialylation motifs correlates with the localized activity of the corresponding glycan biosynthesis pathways. These findings provide a basis for the rational design of zebrafish lines expressing desired glycosylation profiles.

Date: 2018
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DOI: 10.1038/s41467-018-06950-3

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