Anomalous X-ray diffraction studies of ion transport in K+ channels

Patricia S. Langan, Venu Gopal Vandavasi, Kevin L. Weiss, Pavel V. Afonine, Kamel el Omari, Ramona Duman, Armin Wagner and Leighton Coates ()
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Patricia S. Langan: Oak Ridge National Laboratory
Venu Gopal Vandavasi: Oak Ridge National Laboratory
Kevin L. Weiss: Oak Ridge National Laboratory
Pavel V. Afonine: Lawrence Berkeley National Laboratory
Kamel el Omari: Harwell Science and Innovation Campus
Ramona Duman: Harwell Science and Innovation Campus
Armin Wagner: Harwell Science and Innovation Campus
Leighton Coates: Oak Ridge National Laboratory

Nature Communications, 2018, vol. 9, issue 1, 1-5

Abstract: Abstract Potassium ion channels utilize a highly selective filter to rapidly transport K+ ions across cellular membranes. This selectivity filter is composed of four binding sites which display almost equal electron density in crystal structures with high potassium ion concentrations. This electron density can be interpreted to reflect a superposition of alternating potassium ion and water occupied states or as adjacent potassium ions. Here, we use single wavelength anomalous dispersion (SAD) X-ray diffraction data collected near the potassium absorption edge to show experimentally that all ion binding sites within the selectivity filter are fully occupied by K+ ions. These data support the hypothesis that potassium ion transport occurs by direct Coulomb knock-on, and provide an example of solving the phase problem by K-SAD.

Date: 2018
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DOI: 10.1038/s41467-018-06957-w

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