Competing protein-protein interactions regulate binding of Hsp27 to its client protein tau

Rebecca Freilich, Miguel Betegon, Eric Tse, Sue-Ann Mok, Olivier Julien, David A. Agard, Daniel R. Southworth, Koh Takeuchi and Jason E. Gestwicki ()
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Rebecca Freilich: University of California San Francisco
Miguel Betegon: University of California San Francisco
Eric Tse: University of California San Francisco
Sue-Ann Mok: University of California San Francisco
Olivier Julien: University of California San Francisco
David A. Agard: University of California San Francisco
Daniel R. Southworth: University of California San Francisco
Koh Takeuchi: National Institute of Advanced Industrial Science and Technology (AIST)
Jason E. Gestwicki: University of California San Francisco

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract Small heat shock proteins (sHSPs) are a class of oligomeric molecular chaperones that limit protein aggregation. However, it is often not clear where sHSPs bind on their client proteins or how these protein-protein interactions (PPIs) are regulated. Here, we map the PPIs between human Hsp27 and the microtubule-associated protein tau (MAPT/tau). We find that Hsp27 selectively recognizes two aggregation-prone regions of tau, using the conserved β4-β8 cleft of its alpha-crystallin domain. The β4-β8 region is also the site of Hsp27–Hsp27 interactions, suggesting that competitive PPIs may be an important regulatory paradigm. Indeed, we find that each of the individual PPIs are relatively weak and that competition for shared sites seems to control both client binding and Hsp27 oligomerization. These findings highlight the importance of multiple, competitive PPIs in the function of Hsp27 and suggest that the β4-β8 groove acts as a tunable sensor for clients.

Date: 2018
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DOI: 10.1038/s41467-018-07012-4

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