Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

Satoshi Yuzawa (), Mona Mirsiaghi, Renee Jocic, Tatsuya Fujii, Fabrice Masson, Veronica T. Benites, Edward E. K. Baidoo, Eric Sundstrom, Deepti Tanjore, Todd R. Pray, Anthe George, Ryan W. Davis, John M. Gladden, Blake A. Simmons, Leonard Katz and Jay D. Keasling ()
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Satoshi Yuzawa: Lawrence Berkeley National Laboratory
Mona Mirsiaghi: Lawrence Berkeley National Laboratory
Renee Jocic: Lawrence Berkeley National Laboratory
Tatsuya Fujii: Joint BioEnegy Institute
Fabrice Masson: Lawrence Berkeley National Laboratory
Veronica T. Benites: Lawrence Berkeley National Laboratory
Edward E. K. Baidoo: Lawrence Berkeley National Laboratory
Eric Sundstrom: Lawrence Berkeley National Laboratory
Deepti Tanjore: Lawrence Berkeley National Laboratory
Todd R. Pray: Lawrence Berkeley National Laboratory
Anthe George: Joint BioEnegy Institute
Ryan W. Davis: Sandia National Laboratory
John M. Gladden: Joint BioEnegy Institute
Blake A. Simmons: Lawrence Berkeley National Laboratory
Leonard Katz: Joint BioEnegy Institute
Jay D. Keasling: Lawrence Berkeley National Laboratory

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5–C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g · l−1 of C6 and C7 ethyl ketones and several hundred mg · l−1 of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.

Date: 2018
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DOI: 10.1038/s41467-018-07040-0

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