CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides

Huidong Wang, Yu Shi, Lu Wang, Shuai Liu, Shuwen Wu, Yihua Yang, René Feyereisen and Yidong Wu ()
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Huidong Wang: Nanjing Agricultural University
Yu Shi: Nanjing Agricultural University
Lu Wang: Nanjing Agricultural University
Shuai Liu: Nanjing Agricultural University
Shuwen Wu: Nanjing Agricultural University
Yihua Yang: Nanjing Agricultural University
René Feyereisen: University of Copenhagen
Yidong Wu: Nanjing Agricultural University

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract The cotton bollworm Helicoverpa armigera, is one of the world’s major pest of agriculture, feeding on over 300 hosts in 68 plant families. Resistance cases to most insecticide classes have been reported for this insect. Management of this pest in agroecosystems relies on a better understanding of how it copes with phytochemical or synthetic toxins. We have used genome editing to knock out a cluster of nine P450 genes and show that this significantly reduces the survival rate of the insect when exposed to two classes of host plant chemicals and two classes of insecticides. Functional expression of all members of this gene cluster identified the P450 enzymes capable of metabolism of these xenobiotics. The CRISPR-Cas9-based reverse genetics approach in conjunction with in vitro metabolism can rapidly identify the contributions of insect P450s in xenobiotic detoxification and serve to identify candidate genes for insecticide resistance.

Date: 2018
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DOI: 10.1038/s41467-018-07226-6

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