Near-infrared STED nanoscopy with an engineered bacterial phytochrome

Maria Kamper, Haisen Ta, Nickels A. Jensen, Stefan W. Hell and Stefan Jakobs ()
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Maria Kamper: Max Planck Institute for Biophysical Chemistry
Haisen Ta: Max Planck Institute for Biophysical Chemistry
Nickels A. Jensen: Max Planck Institute for Biophysical Chemistry
Stefan W. Hell: Max Planck Institute for Biophysical Chemistry
Stefan Jakobs: Max Planck Institute for Biophysical Chemistry

Nature Communications, 2018, vol. 9, issue 1, 1-7

Abstract: Abstract The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

Date: 2018
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DOI: 10.1038/s41467-018-07246-2

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