Dynamic and non-contact 3D sample rotation for microscopy

Frederic Berndt, Gopi Shah, Rory M. Power, Jan Brugués and Jan Huisken ()
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Frederic Berndt: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Gopi Shah: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Rory M. Power: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Jan Brugués: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Jan Huisken: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108

Nature Communications, 2018, vol. 9, issue 1, 1-7

Abstract: Abstract Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.

Date: 2018
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DOI: 10.1038/s41467-018-07504-3

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