Ribosome-induced RNA conformational changes in a viral 3′-UTR sense and regulate translation levels

Erik W. Hartwick, David A. Costantino, Andrea MacFadden, Jay C. Nix, Siqi Tian, Rhiju Das and Jeffrey S. Kieft ()
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Erik W. Hartwick: University of Colorado Denver School of Medicine
David A. Costantino: University of Colorado Denver School of Medicine
Andrea MacFadden: University of Colorado Denver School of Medicine
Jay C. Nix: Lawrence Berkeley National Laboratory
Siqi Tian: Stanford University
Rhiju Das: Stanford University
Jeffrey S. Kieft: University of Colorado Denver School of Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Structured RNA elements, programmed RNA conformational changes, and interactions between different RNA domains underlie many modes of regulating gene expression, mandating studies to understand the foundational principles that govern these phenomena. Exploring the structured 3′ untranslated region (UTR) of a viral RNA, we discovered that different contexts of the 3′-UTR confer different abilities to enhance translation of an associated open reading frame. In one context, ribosome-induced conformational changes in a ‘sensor’ RNA domain affect a separate RNA ‘functional’ domain, altering translation efficiency. The structure of the entire 3′-UTR reveals that structurally distinct domains use a spine of continuously stacked bases and a strut-like linker to create a conduit for communication within the higher-order architecture. Thus, this 3′-UTR RNA illustrates how RNA can use programmed conformational changes to sense the translation status of an upstream open reading frame, then create a tuned functional response by communicating that information to other RNA elements.

Date: 2018
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DOI: 10.1038/s41467-018-07542-x

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