Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling

Linh T. Vuong, Carlo Iomini (), Sophie Balmer, Davide Esposito, Stuart A. Aaronson and Marek Mlodzik ()
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Linh T. Vuong: Icahn School of Medicine at Mount Sinai
Carlo Iomini: Icahn School of Medicine at Mount Sinai
Sophie Balmer: Icahn School of Medicine at Mount Sinai
Davide Esposito: Icahn School of Medicine at Mount Sinai
Stuart A. Aaronson: Icahn School of Medicine at Mount Sinai
Marek Mlodzik: Icahn School of Medicine at Mount Sinai

Nature Communications, 2018, vol. 9, issue 1, 1-16

Abstract: Abstract Wnt/Wg-signalling is critical signalling in all metazoans. Recent studies suggest that IFT-A proteins and Kinesin-2 modulate canonical Wnt/Wg-signalling independently of their ciliary role. Whether they function together in Wnt-signalling and their mechanistic role in the pathway remained unresolved. Here we demonstrate that Kinesin-2 and IFT-A proteins act as a complex during Drosophila Wg-signalling, affecting pathway activity in the same manner, interacting genetically and physically, and co-localizing with β-catenin, the mediator of Wnt/Wg-signalling on microtubules. Following pathway activation, Kinesin-2/IFT-A mutant cells exhibit high cytoplasmic β-catenin levels, yet fail to activate Wg-targets. In mutant tissues in both, Drosophila and mouse/MEFs, nuclear localization of β-catenin is markedly reduced. We demonstrate a conserved, motor-domain dependent function of the Kinesin-2/IFT-A complex in promoting nuclear translocation of β-catenin. We show that this is mediated by protecting β-catenin from a conserved cytoplasmic retention process, thus identifying a mechanism for Kinesin-2/IFT-A in Wnt-signalling that is independent of their ciliary role.

Date: 2018
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DOI: 10.1038/s41467-018-07605-z

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