TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network

Luciana Renna, Giovanni Stefano, Erin Slabaugh, Clarissa Wormsbaecher, Alan Sulpizio, Krzysztof Zienkiewicz and Federica Brandizzi ()
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Luciana Renna: Michigan State University
Giovanni Stefano: Michigan State University
Erin Slabaugh: Michigan State University
Clarissa Wormsbaecher: Michigan State University
Alan Sulpizio: Michigan State University
Krzysztof Zienkiewicz: Michigan State University
Federica Brandizzi: Michigan State University

Nature Communications, 2018, vol. 9, issue 1, 1-15

Abstract: Abstract Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.

Date: 2018
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DOI: 10.1038/s41467-018-07662-4

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