Monitoring drug nanocarriers in human blood by near-infrared fluorescence correlation spectroscopy

Inka Negwer, Andreas Best, Meike Schinnerer, Olga Schäfer, Leon Capeloa, Manfred Wagner, Manfred Schmidt, Volker Mailänder, Mark Helm, Matthias Barz, Hans-Jürgen Butt () and Kaloian Koynov ()
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Inka Negwer: Max Planck Institute for Polymer Research
Andreas Best: Max Planck Institute for Polymer Research
Meike Schinnerer: Institute of Physical Chemistry, Johannes Gutenberg University
Olga Schäfer: Institute of Organic Chemistry, Johannes Gutenberg University
Leon Capeloa: Institute of Organic Chemistry, Johannes Gutenberg University
Manfred Wagner: Max Planck Institute for Polymer Research
Manfred Schmidt: Institute of Physical Chemistry, Johannes Gutenberg University
Volker Mailänder: Max Planck Institute for Polymer Research
Mark Helm: Pharmaceutical Chemistry, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University
Matthias Barz: Institute of Organic Chemistry, Johannes Gutenberg University
Hans-Jürgen Butt: Max Planck Institute for Polymer Research
Kaloian Koynov: Max Planck Institute for Polymer Research

Nature Communications, 2018, vol. 9, issue 1, 1-9

Abstract: Abstract Nanocarrier-based drug delivery is a promising therapeutic approach that offers unique possibilities for the treatment of various diseases. However, inside the blood stream, nanocarriers’ properties may change significantly due to interactions with proteins, aggregation, decomposition or premature loss of cargo. Thus, a method for precise, in situ characterization of drug nanocarriers in blood is needed. Here we show how the fluorescence correlation spectroscopy that is a well-established method for measuring the size, loading efficiency and stability of drug nanocarriers in aqueous solutions can be used to directly characterize drug nanocarriers in flowing blood. As the blood is not transparent for visible light and densely crowded with cells, we label the nanocarriers or their cargo with near-infrared fluorescent dyes and fit the experimental autocorrelation functions with an analytical model accounting for the presence of blood cells. The developed methodology contributes towards quantitative understanding of the in vivo behavior of nanocarrier-based therapeutics.

Date: 2018
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DOI: 10.1038/s41467-018-07755-0

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