Risk Factors for the Contamination of Wild Stomoxys niger niger Macquart 1851 (Diptera: Muscidae) with the Foot-and-Mouth Disease Virus

Sevidzem Silas Lendzele (), Mamoudou Abdoulmoumini (), Simon Dickmu (), Alfons Renz (), Genevieve Lydie (), Jacques François Mavoungou () and Rebecca Garabed ()

Current Research in Agricultural Sciences, 2019, vol. 6, issue 2, 95-108

Abstract: The study aimed at detecting the foot-and-mouth disease virus (FMDV) RNA in Stomoxys niger niger and cattle. Flies were collected using a Vavoua trap pitched 50m from the center of the herd and by net-catches on clinically sick (n=5) and symptomless cattle (n=5). Vesicular Epithelia Tissues (VETs) from sick cattle were analyzed by real time reverse transcriptase polymerase chain reaction (rt RT PCR) and serum from randomly selected animals without clinical signs was examined by serological test (NSP-ELISA Kit) for the presence of FMDV antibodies. Of a total of 568 Stomoxys trapped using the Vavoua trap, two species were identified namely 196 S. niger niger (34.51 %, 9. 33 Snn/t/d), 101 S. omega (17.78%, 4.80 so/t/d) and 271 non-biting Musca sp. (47.71%, 12.90 m/vavoua and day). The dissected mouth and legs of each fly caught were screened for FMDV using the rt RT PCR. FMDV RNA was found in the epithelial vesicles of all clinically sick animals (n=5, with mean Ct of 27.98456) and 3 out of 5 were serologically positive in the clinically in-apparent cattle group. The overall S. n. niger (most abundant species) contamination rate with the FMDV irrespective of collection method was 40.3 % with females (49.0 %) being slightly more contaminated than males (21.7%) (OR =0.45, P= 0.1506) and legs being more often positive than mouth-parts (P=0.02002). Flies were contaminated less frequently on animals without clinical signs than those on animals with clinical signs, but this difference was not significant (P=0.69680).

Keywords: Stomoxys; legs; Mouth parts; Net-catches; Vavoua; Contamination; FMDV. (search for similar items in EconPapers)
Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://archive.conscientiabeam.com/index.php/68/article/view/120/163 (application/pdf)
https://archive.conscientiabeam.com/index.php/68/article/view/120/2593 (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:pkp:criasc:v:6:y:2019:i:2:p:95-108:id:120

Access Statistics for this article

More articles in Current Research in Agricultural Sciences from Conscientia Beam
Bibliographic data for series maintained by Dim Michael ().