SiCTeC: An inexpensive, easily assembled Peltier device for rapid temperature shifting during single-cell imaging

Benjamin D Knapp, Lillian Zhu and Kerwyn Casey Huang

PLOS Biology, 2020, vol. 18, issue 11, 1-17

Abstract: Single-cell imaging, combined with recent advances in image analysis and microfluidic technologies, have enabled fundamental discoveries of cellular responses to chemical perturbations that are often obscured by traditional liquid-culture experiments. Temperature is an environmental variable well known to impact growth and to elicit specific stress responses at extreme values; it is often used as a genetic tool to interrogate essential genes. However, the dynamic effects of temperature shifts have remained mostly unstudied at the single-cell level, due largely to engineering challenges related to sample stability, heatsink considerations, and temperature measurement and feedback. Additionally, the few commercially available temperature-control platforms are costly. Here, we report an inexpensive (

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pbio00:3000786

DOI: 10.1371/journal.pbio.3000786

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