Active DNA demethylation upstream of rod-photoreceptor fate determination is required for retinal development

Ismael Hernández-Núñez, Alaina Urman, Xiaodong Zhang, William Jacobs, Christy Hoffmann, Ellen G Harding, Shiming Chen, Meelad M Dawlaty, Philip A Ruzycki, John R Edwards and Brian S Clark

PLOS Biology, 2025, vol. 23, issue 8, 1-36

Abstract: Retinal cell fate specification from multipotent retinal progenitors is governed by dynamic changes in chromatin structure and gene expression. Methylation at cytosines in DNA (5mC) is actively regulated for proper control of gene expression and chromatin architecture. Numerous genes display active DNA demethylation across retinal development; a process that requires oxidation of 5mC to 5-hydroxymethylcytosine (5hmC) and is controlled by the ten-eleven translocation (TET) methylcytosine dioxygenase enzymes. Using an allelic series of conditional TET enzyme mutants in mice, we determine that DNA demethylation is required upstream of NRL and NR2E3 expression for the establishment of rod-photoreceptor fate. Using histological, behavioral, transcriptomic, and base-pair resolution DNA methylation analyses, we establish that inhibition of active DNA demethylation results in global changes in gene expression and methylation patterns that prevent photoreceptor precursors from adopting a rod-photoreceptor fate, instead producing a retina in which all photoreceptors specify as cones. Our results establish the TET enzymes and DNA demethylation as critical regulators of retinal development and cell fate specification, elucidating a novel mechanism required for the specification of rod-photoreceptors.TET enzymes remove DNA methylation markers, but the role of this process in retinal development is not clear. This study shows that these enzymes are required for photoreceptor cells to initiate the genetic program to become rods instead of cones, and for maturation of the mouse retina.

Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pbio00:3003332

DOI: 10.1371/journal.pbio.3003332

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