 Defining an Essence of Structure Determining Residue Contacts in ProteinsR Sathyapriya, Jose M Duarte, Henning Stehr, Ioannis Filippis and Michael Lappe PLOS Computational Biology, 2009, vol. 5, issue 12, 1-10 Abstract: The network of native non-covalent residue contacts determines the three-dimensional structure of a protein. However, not all contacts are of equal structural significance, and little knowledge exists about a minimal, yet sufficient, subset required to define the global features of a protein. Characterisation of this “structural essence” has remained elusive so far: no algorithmic strategy has been devised to-date that could outperform a random selection in terms of 3D reconstruction accuracy (measured as the Ca RMSD). It is not only of theoretical interest (i.e., for design of advanced statistical potentials) to identify the number and nature of essential native contacts—such a subset of spatial constraints is very useful in a number of novel experimental methods (like EPR) which rely heavily on constraint-based protein modelling. To derive accurate three-dimensional models from distance constraints, we implemented a reconstruction pipeline using distance geometry. We selected a test-set of 12 protein structures from the four major SCOP fold classes and performed our reconstruction analysis. As a reference set, series of random subsets (ranging from 10% to 90% of native contacts) are generated for each protein, and the reconstruction accuracy is computed for each subset. We have developed a rational strategy, termed “cone-peeling” that combines sequence features and network descriptors to select minimal subsets that outperform the reference sets. We present, for the first time, a rational strategy to derive a structural essence of residue contacts and provide an estimate of the size of this minimal subset. Our algorithm computes sparse subsets capable of determining the tertiary structure at approximately 4.8 Å Ca RMSD with as little as 8% of the native contacts (Ca-Ca and Cb-Cb). At the same time, a randomly chosen subset of native contacts needs about twice as many contacts to reach the same level of accuracy. This “structural essence” opens new avenues in the fields of structure prediction, empirical potentials and docking.Author Summary: A protein structure can be visualized as a network of non-covalent contacts existing between amino acids. But not all such contacts are important structural determinants of a protein. We have attempted to identify a subset of amino acid contacts that are essential for reconstructing protein structures. Initially, we followed random sampling of contacts and tested their efficacy to successfully represent the three-dimensional structure. Further, we also developed an algorithm that selects a subset of amino acid contacts from proteins based on the sequence and network properties. The subsets picked by our algorithm represent protein three-dimensional structure better than random subsets, thereby offering direct evidence for the existence of a structural essence in protein structures. The identification of such structure-defining subsets finds application in experimental and computational protein structure determination. Date: 2009 Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:plo:pcbi00:1000584 DOI: 10.1371/journal.pcbi.1000584 Access Statistics for this article More articles in PLOS Computational Biology from Public Library of Science |
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