Automatic Prediction of Protein 3D Structures by Probabilistic Multi-template Homology Modeling

Armin Meier and Johannes Söding

PLOS Computational Biology, 2015, vol. 11, issue 10, 1-20

Abstract: Homology modeling predicts the 3D structure of a query protein based on the sequence alignment with one or more template proteins of known structure. Its great importance for biological research is owed to its speed, simplicity, reliability and wide applicability, covering more than half of the residues in protein sequence space. Although multiple templates have been shown to generally increase model quality over single templates, the information from multiple templates has so far been combined using empirically motivated, heuristic approaches.We present here a rigorous statistical framework for multi-template homology modeling. First, we find that the query proteins’ atomic distance restraints can be accurately described by two-component Gaussian mixtures. This insight allowed us to apply the standard laws of probability theory to combine restraints from multiple templates. Second, we derive theoretically optimal weights to correct for the redundancy among related templates. Third, a heuristic template selection strategy is proposed.We improve the average GDT-ha model quality score by 11% over single template modeling and by 6.5% over a conventional multi-template approach on a set of 1000 query proteins. Robustness with respect to wrong constraints is likewise improved. We have integrated our multi-template modeling approach with the popular MODELLER homology modeling software in our free HHpred server http://toolkit.tuebingen.mpg.de/hhpred and also offer open source software for running MODELLER with the new restraints at https://bitbucket.org/soedinglab/hh-suite.Author Summary: Since a protein’s function is largely determined by its structure, predicting a protein’s structure from its amino acid sequence can be very useful to understand its molecular functions and its role in biological pathways. By far the most widely used computational approach for protein structure prediction relies on detecting a homologous relationship with a protein of known structure and using this protein as a template to model the structure of the query protein on it. The basic concepts of this homology modelling approach have not changed during the last 20 years. In this study we extend the probabilistic formulation of homology modelling to the consistent treatment of multiple templates. Our new theoretical approach allowed us to improve the quality of homology models by 11% over a baseline single-template approach and by 6.5% over a multi-template approach.

Date: 2015
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Citations: View citations in EconPapers (2)

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Persistent link: https://EconPapers.repec.org/RePEc:plo:pcbi00:1004343

DOI: 10.1371/journal.pcbi.1004343

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