A simulation of the random and directed motion of dendritic cells in chemokine fields

Avery Parr, Nicholas R Anderson and Daniel A Hammer

PLOS Computational Biology, 2019, vol. 15, issue 10, 1-16

Abstract: Dendritic cells (DCs) are the most effective professional antigen-presenting cell. They ferry antigen from the extremities to T cells and are essential for the initiation of an adaptive immune response. Despite interest in how DCs respond to chemical stimuli, there have been few attempts to model DC migration. In this paper, we simulate the motility of DCs by modeling the generation of forces by filopodia and a force balance on the cell. The direction of fliopodial extension is coupled to differential occupancy of cognate chemokine receptors across the cell. Our model simulates chemokinesis and chemotaxis in a variety of chemical and mechanical environments. Simulated DCs undergoing chemokinesis were measured to have a speed of 5.1 ± 0.07 μm·min-1 and a persistence time of 3.2 ± 0.46 min, consistent with experiment. Cells undergoing chemotaxis exhibited a stronger chemotactic response when exposed to lower average chemokine concentrations, also consistent with experiment. We predicted that when placed in two opposing gradients, cells will cluster in a line, which we call the “line of equistimulation;” this clustering has also been observed. We calculated the effect of varying gradient steepness on the line of equistimulation, with steeper gradients resulting in tighter clustering. Moreover, gradients are found to be most potent when cells are in a gradient of chemokine whose mean concentration is close to the binding of the Kd to the receptor, and least potent when the mean concentration is 0.1Kd. Comparing our simulations to experiment, we can give a quantitative measure of the strength of certain chemokines relative to others. Assigning the signal of CCL19 binding CCR7 a baseline strength of 1, we found CCL21 binding CCR7 had a strength of 0.28, and CXCL12 binding CXCR4 had a strength of 0.30. These differences emerge despite both chemokines having virtually the same Kd, suggesting a mechanism of signal amplification in DCs requiring further study.Author summary: Dendritic cells harvest and display antigen to other immune cells, and motility is essential to their function. Dendritic cells use filopodia to pull themselves forward, and orient their filopodia based on signals received from chemokines. We developed a model of dendritic cell motion based on a force balance, in which pulling from filopodia (calculated from a well-established model of filopodial adhesion-clutch dynamics) is counterbalanced by adhesive friction, and where the angular orientation of filopodia is based on the strength of chemotactic signal. The model can explain directional motion in chemotactic gradients, as well as the effect of superposition of coincident gradients of chemokine on chemotaxis. The model also identifies conditions in which dendritic cells can be focused at a single location when confronted with countergradients of chemokine. The model makes specific prediction regarding dendritic cell motion and defines conditions for the design of extracorporeal devices for focusing the position of dendritic cells.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pcbi00:1007295

DOI: 10.1371/journal.pcbi.1007295

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