Signal-to-signal neural networks for improved spike estimation from calcium imaging data

Jilt Sebastian, Mriganka Sur, Hema A Murthy and Mathew Magimai-Doss

PLOS Computational Biology, 2021, vol. 17, issue 3, 1-19

Abstract: Spiking information of individual neurons is essential for functional and behavioral analysis in neuroscience research. Calcium imaging techniques are generally employed to obtain activities of neuronal populations. However, these techniques result in slowly-varying fluorescence signals with low temporal resolution. Estimating the temporal positions of the neuronal action potentials from these signals is a challenging problem. In the literature, several generative model-based and data-driven algorithms have been studied with varied levels of success. This article proposes a neural network-based signal-to-signal conversion approach, where it takes as input raw-fluorescence signal and learns to estimate the spike information in an end-to-end fashion. Theoretically, the proposed approach formulates the spike estimation as a single channel source separation problem with unknown mixing conditions. The source corresponding to the action potentials at a lower resolution is estimated at the output. Experimental studies on the spikefinder challenge dataset show that the proposed signal-to-signal conversion approach significantly outperforms state-of-the-art-methods in terms of Pearson’s correlation coefficient, Spearman’s rank correlation coefficient and yields comparable performance for the area under the receiver operating characteristics measure. We also show that the resulting system: (a) has low complexity with respect to existing supervised approaches and is reproducible; (b) is layer-wise interpretable, and (c) has the capability to generalize across different calcium indicators.Author summary: Information processing by a population of neurons is studied using two-photon calcium imaging techniques. A neuronal spike results in an increased intracellular calcium concentration. Fluorescent calcium indicators change their brightness upon a change in the calcium concentration, and this change is captured in the imaging technique. The task of estimating the actual spike positions from the brightness variations is formally referred to as spike estimation. Several signal processing and machine learning-based algorithms have been proposed in the past to solve this problem. However, the task is still far from being solved. Here we present a novel neural network-based data-driven algorithm for spike estimation. Our method takes the fluorescence recording as the input and synthesizes the spike information signal, which is well-correlated with the actual spike positions. Our method outperforms state-of-the-art methods on a standard evaluation framework. We further analyze different components of the model and discuss its benefits.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pcbi00:1007921

DOI: 10.1371/journal.pcbi.1007921

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