Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria

Ernest L Yufenyuy, Olusola A Akanbi, Vedapuri Shanmugam, Kelsie Decker-Pulice, Jeni Vuong, Mervi Detorio, Amy Zheng, Orji Bassey, Ado G Abubakar, Oluwaseun Akinmulero, Mudiaga Esiekpe, Andrew Thomas, Iliyasu Abubakar Bichi, Israel Tamunonengiyeofori, Chinwe Ugwu, Evbuomwan Erasogie, William Nwachukwu, Nwando Mba, Ndidi Agala, Megan Bronson, Hetal K Patel, Nnaemeka C Iriemenem, Stacie Greby, McPaul I Okoye, Mahesh Swaminathan, Bharat S Parekh and Chikwe Ihekweazu

PLOS Global Public Health, 2025, vol. 5, issue 4, 1-13

Abstract: HIV cross-sectional surveys require multi-layered testing with several tests to estimate HIV prevalence and HIV-1 incidence. We evaluated the performance and accuracy of the newly developed HIV Triplex assay to diagnose HIV-1 and HIV-2 and detect HIV-1 recent infections using plasma samples from the 2018 Nigeria AIDS Indicator and Impact Survey (NAIIS). Plasma samples from consenting HIV-positive (n=2,773) and a subset of HIV-negative samples (n=7,196), as determined by the national rapid testing algorithm, followed by Bio-Rad Geenius HIV-1/2 Supplemental Assay and Western Blot, aged 18 months - 64 years, were tested using the Luminex-based HIV Triplex assay. The assay classified specimens as HIV-1 positive, HIV-2 positive, dual (HIV-1 & 2) infections, or HIV-seronegative. All HIV-1 and dual infections were further classified as either HIV-1 recent (

Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pgph00:0003455

DOI: 10.1371/journal.pgph.0003455

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