Differential expression profile of genes involved in the immune response associated to progression of chronic Chagas disease

Inmaculada Gómez, Manuel Carlos López, Adriana Egui, Génesis Palacios, Bartolomé Carrilero, Celia Benítez, Marina Simón, Manuel Segovia, Emma Carmelo and M Carmen Thomas

PLOS Neglected Tropical Diseases, 2023, vol. 17, issue 7, 1-25

Abstract: Background: Patients with chronic Chagas disease present marked clinical and immunological heterogeneity. During the disease, multiple immune mechanisms are activated to fight the parasite. The purpose of this study was to investigate the expression patterns of genes involved in relevant immunological processes throughout the disease in patients with chronic Chagas disease. Methodology/Principal findings: High-throughput RT-qPCR with QuantStudio 12K Flex real-time PCR system was used to evaluate the expression of 106 immune-related genes in PBMC from a cohort of cardiac Chagas disease patients (CCC I), asymptomatic patients (IND) and healthy donors (HD) after being stimulated with T. cruzi soluble antigens. Principal component analysis (PCA), cluster analysis and volcano plots were used to identify differentially expressed genes. In addition, gene set enrichment analysis (GSEA) was employed to identify the enriched immunological pathways in which these genes are involved. PCA revealed the existence of a statistically divergent expression profile of the 36 genes correlated with PC1 between CCC I patients and HD (p 1.5) and downregulation of 14 genes (expression fold-change

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pntd00:0011474

DOI: 10.1371/journal.pntd.0011474

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