Two Forms of Activation-Induced Cytidine Deaminase Differing in Their Ability to Bind Agarose

Mirjam Metzner, Wolfgang Schuh, Edith Roth, Hans-Martin Jäck and Matthias Wabl

PLOS ONE, 2010, vol. 5, issue 1, 1-6

Abstract: Background: Activation-induced cytidine deaminase (AID) is a B-cell-specific DNA mutator that plays a key role in the formation of the secondary antibody repertoire in germinal center B cells. In the search for binding partners, protein coimmunoprecipitation assays are often performed, generally with agarose beads. Methodology/Principal Findings: We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouse AID forms bound to agarose alone. Conclusions/Significance: These binding characteristics may be due to the known post-translational modifications of AID; they may also need to be considered in coimmunoprecipitation experiments to avoid false-positive results.

Date: 2010
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0008883

DOI: 10.1371/journal.pone.0008883

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