High-Content, Image-Based Screening for Drug Targets in Yeast

Shinsuke Ohnuki, Satomi Oka, Satoru Nogami and Yoshikazu Ohya

PLOS ONE, 2010, vol. 5, issue 4, 1-11

Abstract: Background: Drug discovery and development are predicated on elucidation of the potential mechanisms of action and cellular targets of candidate chemical compounds. Recent advances in high-content imaging techniques allow simultaneous analysis of a range of cellular events. In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging in yeast. Methodology: In this approach, we infer the cellular functions affected by candidate drugs by comparing morphologic changes induced by the compounds with the phenotypes of yeast mutants. Conclusions: Using this method and four well-characterized reagents, we successfully identified previously known target genes of the compounds as well as other genes involved with functionally related cellular pathways. This is the first demonstration of a genetic high-content assay that can be used to identify drug targets based on morphologic phenotypes of a reference mutant panel.

Date: 2010
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0010177

DOI: 10.1371/journal.pone.0010177

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