The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

Narumi Ogonuki, Manami Mori, Akie Shinmen, Kimiko Inoue, Keiji Mochida, Akihiko Ohta and Atsuo Ogura

PLOS ONE, 2010, vol. 5, issue 6, 1-7

Abstract: Background: Intracytoplasmic sperm injection (ICSI) has been widely used to study the mechanisms of mammalian fertilization and to rescue male-factor infertility in humans and animals. However, very few systematic analyses have been conducted to define factors affecting the efficiency of ICSI. In this study, we undertook a large-scale series of ICSI experiments in mice to define the factors that might affect outcomes. Methodology/Principal Findings: We used a 5×3×2 factorial design with the following factors: mouse genotype (ICR, C57BL/6, DBA/2, C3H/He, and 129/Sv strains), type of male germ cells (epididymal sperm, elongated or round spermatids), and their freeze–thawing treatment. The efficiencies (parameters) of each developmental step were analyzed by three-way ANOVA (significance level P

Date: 2010
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0011062

DOI: 10.1371/journal.pone.0011062

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