Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers

Yu-Wei Chiu, Men Tzung Lo, Ming-Rung Tsai, Yi-Chung Chang, Rong-Bin Hsu, Hsu-Yu Yu, Chi-Kuang Sun and Yi-Lwun Ho

PLOS ONE, 2010, vol. 5, issue 11, 1-9

Abstract: Background: Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated. Objective: This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach. Patients and Methods: Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image texture was constituted and its entropy was used to quantify the mal-alignment of collagen fibers. The amount of collagen fiber was derived from its area ratio to total atrial tissue in each image. Serum C-terminal pro-collagen pro-peptide (CICP), pro-matrix metalloproteinase-1 (pro-MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were also evaluated. Results: 46 patients were evaluated, including 20 with normal sinus rhythm and 26 with AF. The entropy of spectral-energy distribution of collagen alignment was significantly higher in AF than that in sinus rhythm (3.97±0.33 vs. 2.80±0.18, p

Date: 2010
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0013917

DOI: 10.1371/journal.pone.0013917

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