A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis

Marcelo A Lima, Timothy R Rudd, Eduardo H C de Farias, Lyvia F Ebner, Tarsis F Gesteira, Lauro M de Souza, Aline Mendes, Carolina R Córdula, João R M Martins, Debra Hoppensteadt, Jawed Fareed, Guilherme L Sassaki, Edwin A Yates, Ivarne L S Tersariol and Helena B Nader

PLOS ONE, 2011, vol. 6, issue 1, 1-9

Abstract: The year 2007 was marked by widespread adverse clinical responses to heparin use, leading to a global recall of potentially affected heparin batches in 2008. Several analytical methods have since been developed to detect impurities in heparin preparations; however, many are costly and dependent on instrumentation with only limited accessibility. A method based on a simple UV-scanning assay, combined with principal component analysis (PCA), was developed to detect impurities, such as glycosaminoglycans, other complex polysaccharides and aromatic compounds, in heparin preparations. Results were confirmed by NMR spectroscopy. This approach provides an additional, sensitive tool to determine heparin purity and safety, even when NMR spectroscopy failed, requiring only standard laboratory equipment and computing facilities.

Date: 2011
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015970 (text/html)
https://journals.plos.org/plosone/article/file?id= ... 15970&type=printable (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0015970

DOI: 10.1371/journal.pone.0015970

Access Statistics for this article

More articles in PLOS ONE from Public Library of Science
Bibliographic data for series maintained by plosone ().