Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

Paolo Annibale, Stefano Vanni, Marco Scarselli, Ursula Rothlisberger and Aleksandra Radenovic

PLOS ONE, 2011, vol. 6, issue 7, 1-8

Abstract: In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.

Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0022678

DOI: 10.1371/journal.pone.0022678

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