Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation

Yinzhi Lai, Amish Asthana, Ke Cheng and William S Kisaalita

PLOS ONE, 2011, vol. 6, issue 10, 1-8

Abstract: Cells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promising alternative for use in cell-based assays or biosensors in non-clinical drug discovery studies. To advance 3D culture technology, a case has been made for identifying and validating three-dimensionality biomarkers. With this goal in mind, we conducted a transcriptomic expression comparison among neural progenitor cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and as 3D neurospheres (in vivo surrogate). Up-regulation of cytokines as a group in 3D and neurospheres was observed. A group of 13 cytokines were commonly up-regulated in cells cultured in polystyrene scaffolds and neurospheres, suggesting potential for any or a combination from this list to serve as three-dimensionality biomarkers. These results are supportive of further cytokine identification and validation studies with cells from non-neural tissue.

Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0026821

DOI: 10.1371/journal.pone.0026821

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