The Reproducibility of 31-Phosphorus MRS Measures of Muscle Energetics at 3 Tesla in Trained Men

Lindsay M Edwards, Damian J Tyler, Graham J Kemp, Renee M Dwyer, Andrew Johnson, Cameron J Holloway, Alan M Nevill and Kieran Clarke

PLOS ONE, 2012, vol. 7, issue 6, 1-7

Abstract: Objective: Magnetic resonance spectroscopy (MRS) provides an exceptional opportunity for the study of in vivo metabolism. MRS is widely used to measure phosphorus metabolites in trained muscle, although there are no published data regarding its reproducibility in this specialized cohort. Thus, the aim of this study was to assess the reproducibility of 31P-MRS in trained skeletal muscle. Methods: We recruited fifteen trained men (VO2peak = 4.7±0.8 L min−1/58±8 mL kg−1 min−1) and performed duplicate MR experiments during plantar flexion exercise, three weeks apart. Results: Measures of resting phosphorus metabolites were reproducible, with 1.7 mM the smallest detectable difference in phosphocreatine (PCr). Measures of metabolites during exercise were less reliable: exercising PCr had a coefficient of variation (CV) of 27% during exercise, compared with 8% at rest. Estimates of mitochondrial function were variable, but experimentally useful. The CV of PCr1/2t was 40%, yet much of this variance was inter-subject such that differences of

Date: 2012
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0037237 (text/html)
https://journals.plos.org/plosone/article/file?id= ... 37237&type=printable (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0037237

DOI: 10.1371/journal.pone.0037237

Access Statistics for this article

More articles in PLOS ONE from Public Library of Science
Bibliographic data for series maintained by plosone ().