T7 RNA Polymerase Functions In Vitro without Clustering

Kieran Finan, Joseph P Torella, Achillefs N Kapanidis and Peter R Cook

PLOS ONE, 2012, vol. 7, issue 7, 1-9

Abstract: Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a Kd

Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0040207

DOI: 10.1371/journal.pone.0040207

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