The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences

W Martijn ter Haar, Magda A Meester-Smoor, Karel H M van Wely, Claudia C M M Schot, Marjolein J F W Janssen, Bart Geverts, Jacqueline Bonten, Gerard C Grosveld, Adriaan B Houtsmuller and Ellen C Zwarthoff

PLOS ONE, 2012, vol. 7, issue 9, 1-7

Abstract: The leukemia-associated fusion protein MN1-TEL combines the transcription-activating domains of MN1 with the DNA-binding domain of the transcriptional repressor TEL. Quantitative photobleaching experiments revealed that ∼20% of GFP-tagged MN1 and TEL is transiently immobilised, likely due to indirect or direct DNA binding, since transcription inhibition abolished immobilisation. Interestingly, ∼50% of the MN1-TEL fusion protein was immobile with much longer binding times than unfused MN1 and TEL. MN1-TEL immobilisation was not observed when the TEL DNA-binding domain was disrupted, suggesting that MN1-TEL stably occupies TEL recognition sequences, preventing binding of factors required for proper transcription regulation, which may contribute to leukemogenesis.

Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0046085

DOI: 10.1371/journal.pone.0046085

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