Optimization of Fermentation Conditions and Properties of an Exopolysaccharide from Klebsiella sp. H-207 and Application in Adsorption of Hexavalent Chromium

Li Qiang, Li Yumei, Han Sheng, Liu Yingzi, Song Dongxue, Hao Dake, Wang Jiajia, Qu Yanhong and Zheng Yuxia

PLOS ONE, 2013, vol. 8, issue 1, 1-11

Abstract: The novel exopolysaccharide HZ-7 is produced by Klebsiella sp. H-207, and its fermentation conditions were optimized by response surface methodology (RSM). In this study, the optimized medium consisted of sucrose 31.93 g/L, KNO3 2.17 g/L and K2HPO4 5.47 g/L; while the optimized culture conditions consisted of seed age 13 h, with an inoculum size of 10.6% and incubation temperature of 28.9°C. A maximum HZ-7 yield of about 15.05 g/L was achieved under the optimized conditions using RSM and single-factor experiments. Next the exopolysaccharide HZ-7 was partially purified and characterized. The resulting product showed good properties, such as high concentration of uronic acid (41.67%), low average molecular weight (about 1.94×105 Da) and porous surface structure, were very advantageous to biosorption. Therefore HZ-7 was applied to absorb hexavalent chromium (Cr(VI)). The maximum adsorption efficiency (99.2%) which was obtained at an initial pH of 1.0 along with an initial Cr(VI) concentration of 20 mg/L, was not affected by ordinary metal ions and temperature. These data suggest Klebsiella sp. H-207 exopolysaccharide will be promising potential for industrial application.

Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0053542

DOI: 10.1371/journal.pone.0053542

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