Impact of Extraction Parameters on the Recovery of Lipolytic Activity from Fermented Babassu Cake

Jaqueline N Silva, Mateus G Godoy, Melissa L E Gutarra and Denise M G Freire

PLOS ONE, 2014, vol. 9, issue 8, 1-9

Abstract: Enzyme extraction from solid matrix is as important step in solid-state fermentation to obtain soluble enzymes for further immobilization and application in biocatalysis. A method for the recovery of a pool of lipases from Penicillium simplicissimum produced by solid-state fermentation was developed. For lipase recovery different extraction solution was used and phosphate buffer containing Tween 80 and NaCl showed the best results, yielding lipase activity of 85.7 U/g and 65.7 U/g, respectively. The parameters with great impacts on enzyme extraction detected by the Plackett-Burman analysis were studied by Central Composite Rotatable experimental designs where a quadratic model was built showing maximum predicted lipase activity (160 U/g) at 25°C, Tween 80 0.5% (w/v), pH 8.0 and extraction solution 7 mL/g, maintaining constant buffer molarity of 0.1 M and 200 rpm. After the optimization process a 2.5 fold increase in lipase activity in the crude extract was obtained, comparing the intial value (64 U/g) with the experimental design (160 U/g), thus improving the overall productivity of the process.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0103176

DOI: 10.1371/journal.pone.0103176

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