Recalibration of the Limiting Antigen Avidity EIA to Determine Mean Duration of Recent Infection in Divergent HIV-1 Subtypes

Yen T Duong, Reshma Kassanjee, Alex Welte, Meade Morgan, Anindya De, Trudy Dobbs, Erin Rottinghaus, John Nkengasong, Marcel E Curlin, Chonticha Kittinunvorakoon, Boonyos Raengsakulrach, Michael Martin, Kachit Choopanya, Suphak Vanichseni, Yan Jiang, Maofeng Qiu, Haiying Yu, Yan Hao, Neha Shah, Linh-Vi Le, Andrea A Kim, Tuan Anh Nguyen, William Ampofo and Bharat S Parekh

PLOS ONE, 2015, vol. 10, issue 2, 1-15

Abstract: Background: Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus. Methods: A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs. Results: Using different statistical methods, MDRI values ranged from 88–94 days at cutoff ODn = 1.0 to 177–183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0114947

DOI: 10.1371/journal.pone.0114947

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