Quality Control Test for Sequence-Phenotype Assignments

Maria Teresa Lara Ortiz, Pablo Benjamín Leon Rosario, Pablo Luna-Nevarez, Alba Savin Gamez, Ana Martínez-del Campo and Gabriel Del Rio

PLOS ONE, 2015, vol. 10, issue 2, 1-16

Abstract: Relating a gene mutation to a phenotype is a common task in different disciplines such as protein biochemistry. In this endeavour, it is common to find false relationships arising from mutations introduced by cells that may be depurated using a phenotypic assay; yet, such phenotypic assays may introduce additional false relationships arising from experimental errors. Here we introduce the use of high-throughput DNA sequencers and statistical analysis aimed to identify incorrect DNA sequence-phenotype assignments and observed that 10–20% of these false assignments are expected in large screenings aimed to identify critical residues for protein function. We further show that this level of incorrect DNA sequence-phenotype assignments may significantly alter our understanding about the structure-function relationship of proteins. We have made available an implementation of our method at http://bis.ifc.unam.mx/en/software/chispas.

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0118288

DOI: 10.1371/journal.pone.0118288

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