Micro-CT Technique Is Well Suited for Documentation of Remodeling Processes in Murine Carotid Arteries

Christoph Schürmann, Felix Gremse, Hanjoong Jo, Fabian Kiessling and Ralf P Brandes

PLOS ONE, 2015, vol. 10, issue 6, 1-11

Abstract: Background: The pathomechanisms of atherosclerosis and vascular remodelling are under intense research. Only a few in vivo tools to study these processes longitudinally in animal experiments are available. Here, we evaluated the potential of micro-CT technology. Methods: Lumen areas of the common carotid arteries (CCA) in the ApoE-/- partial carotid artery ligation mouse model were compared between in vivo and ex vivo micro-CT technique and serial histology in a total of 28 animals. AuroVist-15 nm nanoparticles were used as in vivo blood pool contrast agent in a Skyscan 1176 micro-CT at resolution of 18 μmeter voxel size and a mean x-ray dose of 0.5 Gy. For ex vivo imaging, animals were perfused with MicroFil and imaged at 9 μmeter voxel size. Lumen area was evaluated at postoperative days 7, 14, and 28 first by micro-CT followed by histology. Results: In vivo micro-CT and histology revealed lumen loss starting at day 14. The lumen profile highly correlated (r = 0.79, P

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0130374

DOI: 10.1371/journal.pone.0130374

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