Cholangiocarcinoma: Correlation between Molecular Profiling and Imaging Phenotypes

Eran Sadot, Amber L Simpson, Richard K G Do, Mithat Gonen, Jinru Shia, Peter J Allen, Michael I D’Angelica, Ronald P DeMatteo, T Peter Kingham and William R Jarnagin

PLOS ONE, 2015, vol. 10, issue 7, 1-12

Abstract: Purpose: To investigate associations between imaging features of cholangiocarcinoma by visual assessment and texture analysis, which quantifies heterogeneity in tumor enhancement patterns, with molecular profiles based on hypoxia markers. Methods: The institutional review board approved this HIPAA-compliant retrospective study of CT images of intrahepatic cholangiocarcinoma, obtained before surgery. Immunostaining for hypoxia markers (EGFR, VEGF, CD24, P53, MDM2, MRP-1, HIF-1α, CA-IX, and GLUT1) was performed on pre-treatment liver biopsies. Quantitative imaging phenotypes were determined by texture analysis with gray level co-occurrence matrixes. The correlations between quantitative imaging phenotypes, qualitative imaging features (measured by radiographic inspection alone), and expression levels of the hypoxia markers from the 25 tumors were assessed. Results: Twenty-five patients were included with a median age of 62 years (range: 54–84). The median tumor size was 10.2 cm (range: 4–14), 10 (40%) were single tumors, and 90% were moderately differentiated. Positive immunostaining was recorded for VEGF in 67% of the cases, EGFR in 75%, and CD24 in 55%. On multiple linear regression analysis, quantitative imaging phenotypes correlated significantly with EGFR and VEGF expression levels (R2 = 0.4, p

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0132953

DOI: 10.1371/journal.pone.0132953

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