Metabolic Analysis of Medicinal Dendrobium officinale and Dendrobium huoshanense during Different Growth Years

Qing Jin, Chunyan Jiao, Shiwei Sun, Cheng Song, Yongping Cai, Yi Lin, Honghong Fan and Yanfang Zhu

PLOS ONE, 2016, vol. 11, issue 1, 1-17

Abstract: Metabolomics technology has enabled an important method for the identification and quality control of Traditional Chinese Medical materials. In this study, we isolated metabolites from cultivated Dendrobium officinale and Dendrobium huoshanense stems of different growth years in the methanol/water phase and identified them using gas chromatography coupled with mass spectrometry (GC-MS). First, a metabolomics technology platform for Dendrobium was constructed. The metabolites in the Dendrobium methanol/water phase were mainly sugars and glycosides, amino acids, organic acids, alcohols. D. officinale and D. huoshanense and their growth years were distinguished by cluster analysis in combination with multivariate statistical analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Eleven metabolites that contributed significantly to this differentiation were subjected to t-tests (P

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0146607

DOI: 10.1371/journal.pone.0146607

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