Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence

Zachary J Farino, Travis J Morgenstern, Julie Vallaghe, Nathalie Gregor, Prashant Donthamsetti, Paul E Harris, Nicolas Pierre, Robin Freyberg, Fabienne Charrier-Savournin, Jonathan A Javitch and Zachary Freyberg

PLOS ONE, 2016, vol. 11, issue 2, 1-17

Abstract: Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0148684

DOI: 10.1371/journal.pone.0148684

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