APNG as a prognostic marker in patients with glioblastoma

Sigurd Fosmark, Sofie Hellwege, Rikke H Dahlrot, Kristian L Jensen, Helene Derand, Jesper Lohse, Mia D Sørensen, Steinbjørn Hansen and Bjarne W Kristensen

PLOS ONE, 2017, vol. 12, issue 6, 1-17

Abstract: Aim: Expression of the base excision repair enzyme alkylpurine-DNA-N-glycosylase (APNG) has been correlated to temozolomide resistance. Our aim was to evaluate the prognostic value of APNG in a population-based cohort with 242 gliomas including 185 glioblastomas (GBMs). Cellular heterogeneity of GBMs was taken into account by excluding APNG expression in non-tumor cells from the analysis. Methods: APNG expression was evaluated using automated image analysis and a novel quantitative immunohistochemical (IHC) assay (qIHC), where APNG protein expression was evaluated through countable dots. Non-tumor cells were excluded using an IHC/qIHC double-staining. For verification, APNG was measured by a quantitative double-immunofluorescence (IF) assay. As validation APNG mRNA expression was evaluated using independent TCGA data. Results: Using qIHC, high levels of APNG were associated with better overall survival (OS) in univariate (HR = 0.50; P

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0178693

DOI: 10.1371/journal.pone.0178693

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