NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways

Qin Lin, Xiaolin Kang, Xuefeng Li, Tao Wang, Fengting Liu, Jinxue Jia, Ziqi Jin and Yongzhi Xue

PLOS ONE, 2019, vol. 14, issue 12, 1-17

Abstract: Cytochrome P450 2E1 (CYP2E1) plays an important role in both alcohol-induced and immune-mediated liver injury. However, the mechanism underlying CYP2E1 transcriptional regulation has not been clarified. This study focused on the NF-κB-mediated transcriptional regulation of rat CYP2E1 by two independent signaling pathways in alcohol-induced and immune-mediated liver injury rat models. Male Sprague-Dawley rats were used in pharmacokinetic, molecular pharmacology, and morphology experiments. A rat model of alcohol-induced liver injury (AL) was established by feeding an ethanol-containing diet (42 g/kg/day) for 5 weeks as indicated. A rat immune-mediated liver injury (IM) model was established by the sequential injection of bacillus Calmette-Guérin (BCG, 125 mg/kg, once) via the tail vein after test day 21 and 10 μg/kg LPS 13 days later. HPLC, real-time PCR, western blot and ELISA analyses were performed. CYP2E1 expression was enhanced during the process of alcohol-induced liver injury (increased by 56%, P

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0225531

DOI: 10.1371/journal.pone.0225531

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