Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system

Naoki Yamamoto, Jiro Kikuchi, Yusuke Furukawa and Naoya Shibayama

PLOS ONE, 2022, vol. 17, issue 5, 1-6

Abstract: We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 kinase inhibitors, and succeeded in screening two inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0261699

DOI: 10.1371/journal.pone.0261699

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