Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex

George S Brush

PLOS ONE, 2022, vol. 17, issue 12, 1-19

Abstract: A G-rich DNA sequence from yeast that can form a non-canonical G-quadruplex structure was cloned into a plasmid vector and subjected to Sanger sequencing using dye-labeled dideoxynucleotides. Two different effects were observed. In one, presence of the G4 sequence on the template strand led to incorrect incorporation of an A residue at an internal position in the G4 sequence. In the other, the nascent strand caused attenuation of the readout coincident with synthesis of the G-rich DNA. The two effects are novel examples of disruption in DNA synthesis caused by a G4 sequence. These results provide a new example of a DNA structure that could influence genomic stability in human cells.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0279423

DOI: 10.1371/journal.pone.0279423

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