Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19

Kamonthip Rungrojcharoenkit, Rungarun Suthangkornkul, Darunee Utennam, Darunee Buddhari, Soontorn Pinpaiboon, Duangrat Mongkolsirichaikul, Stefan Fernandez, Anthony R Jones, Thomas S Cotrone and Taweewun Hunsawong

PLOS ONE, 2023, vol. 18, issue 6, 1-9

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0287107

DOI: 10.1371/journal.pone.0287107

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