A computer vision based optical method for measuring fluid level in cell culture plates

Pierre V Baudin and Mircea Teodorescu

PLOS ONE, 2023, vol. 18, issue 9, 1-12

Abstract: For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0290951

DOI: 10.1371/journal.pone.0290951

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