The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration

Robert Harper, Benedikt Ley, Md Alamgir Kabir, Graham Matulis, Lorenz von Seidlein, Mohammad Shafiul Alam, Bipin Adhikari, Bernard A Okech, Alan L Williams, Ric N Price and Michael E von Fricken

PLOS ONE, 2024, vol. 19, issue 5, 1-10

Abstract: Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.

Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0297918

DOI: 10.1371/journal.pone.0297918

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