PMA-qPCA: Accelerating the market release of high-quality Bradyrhizobium diazoefficiens inoculant

Mariana Cap, Camila Frydman, Antonella Galiñanes, Camila Aranguiz, Irina Faraco, Luisina Andriolo, Viviana Parreño and Marina Mozgovoj

PLOS ONE, 2025, vol. 20, issue 10, 1-12

Abstract: Traditional culture-based quantification of Bradyrhizobium diazoefficiens in inoculants presents significant limitations due to its labor-intensive and time-consuming nature. To address this limitation, we aimed to validate a propidium monoazide quantitative PCR (PMA-qPCR) assay as a rapid and reliable alternative for estimating Bradyrhizobium diazoefficiens counts in commercial inoculants. Key experiments optimized PMA concentration (50, 75 and 100 µM) to selectively inhibit DNA amplification from non-viable cells without interfering with viable cell signal. Assay´s efficiency, limit of detection and quantification, intra-assay repeatability and inter-assay reproducibility were determined. The assay demonstrated high efficiency (90–105%), a limit of detection (LOD) of 3.14 log CFU/mL, and a dynamic range from 8.74 to 3.14 log CFU/mL. Robust intra-assay repeatability (SD

Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0325878

DOI: 10.1371/journal.pone.0325878

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