Effect of ethyl methane sulfonate mutagenesis on phenological, yield-related and yield traits in cowpea (Vigna unguiculata (L.) Walp)

Henry K Mensah, Ralieva A K Nortey, Isaac K Asante and Felicia Oppong-Adjei

PLOS ONE, 2026, vol. 21, issue 7, 1-32

Abstract: This study investigated the mutagenic effects of ethyl methane sulfonate (EMS) on the M₁ generation of cowpea (Vigna unguiculata (L.) Walp.) cultivar ‘Wang Kae.’ A total of 275 M₀ seeds were treated with EMS concentrations of 20 mM, 40 mM, and 80 mM (75 seeds per treatment) by soaking for six hours, while 50 untreated seeds served as the control (0 mM). Phenological, yield-related and yield traits were recorded, and data were analysed using Jamovi 2.7.15 and JASP 0.95.4.0 through one-way ANOVA with post hoc contrast, principal component biplot, and cluster analyses. No optimal mutagenic concentration (LD50) was identified. Seed germination and seedling survival rates increased with increasing EMS concentration, ranging from 70.00% and 62.00% in the control (0 mM) to 89.33% and 74.67% at 80 mM, following the trend 0 mM

Date: 2026
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0354037

DOI: 10.1371/journal.pone.0354037

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