Short-term effects of different toothpaste formulations on total salivary calcium: A randomized crossover study

Lina Aminy, Mozhgan Bizhang, Claudia Klinger, Stefan Zimmer and Andreas Savelsbergh

PLOS ONE, 2026, vol. 21, issue 9, 1-15

Abstract: Objectives: Dental calculus formation can be inhibited by toothpastes containing pyrophosphate, which chelates calcium ions and prevents hydroxyapatite crystal formation. Although pyrophosphate may also influence salivary calcium availability, the short-term effects of commercially available toothpaste formulations on salivary calcium concentrations remain insufficiently characterized. This study investigated and compared the short-term effects of three fluoride toothpastes on salivary calcium concentration. Methods: A randomized, three-treatment, three-period crossover trial was conducted in 48 healthy volunteers. The participants used three commercially available sodium fluoride toothpastes in randomized order: a formulation containing methyl vinyl ether/maleic acid copolymer, PVM/MA copolymer (Sensodyne), a formulation containing pyrophosphate and zinc (Dentalux), and a formulation containing sodium bicarbonate (Parodontax). Treatment periods were separated by a 2-day washout. Unstimulated saliva was collected at baseline (T0), immediately after brushing (T1), 30 min (T2), and 60 min (T3). Salivary calcium concentrations were quantified using a colorimetric assay. Data were analysed using linear mixed-effects models with toothpaste formulation, measurement time, treatment sequence, and the toothpaste-by-time interaction as fixed effects and participant as a random effect. Results: Significant effects were observed for toothpaste formulation (F(2,103.35)=42.31, P

Date: 2026
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0358715

DOI: 10.1371/journal.pone.0358715

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