Effect of carbon-to-nitrogen ratio on eco-friendly mycoherbicide activity from Lasiodiplodia pseudotheobromae C1136 for sustainable weeds management in organic agriculture

Charles Oluwaseun Adetunji (), Julius Kola Oloke and Gandham Prasad
Additional contact information
Charles Oluwaseun Adetunji: Landmark University
Julius Kola Oloke: Ladoke Akintola University of Technology
Gandham Prasad: Institute of Microbial Technology

Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, 2020, vol. 22, issue 3, No 14, 1977-1990

Abstract: Abstract The utilization of bioherbicides in place of chemical herbicides has been described as one of the sustainable eco-friendly technologies that can improve agricultural outputs. This work investigated the impact of different carbon and nitrogen sources on the bioherbicidal efficiency of Lasiodiplodia pseudotheobromae (C1136). The preliminary screening of various carbon and nitrogen sources was carried out, out of which sucrose and peptone were selected for further C:N ratio investigations using strain C1136. The fungus produces a different quantity of mycelium biomass and spores in varied ratios of C:N when compared to the basal medium. Also, the effect of C:N ratio was tested by evaluating for leaf necrosis assay, mortality rate and the dry-matter biomass from the aerial parts of Chromolaena odoratum and Echinochloa crus-galli under guided laboratory and screen house conditions. Both the 7.5:1 and 10:1 ratios produced optimal spores count of 6.1 × 107 and 5.9 × 107 CFU/mL, respectively (p = 0.05) when compared to the control containing potatoes dextrose broth without any carbon or nitrogen amendment produced a spores count of 1.1 × 107 CFU/mL. Also, the phytotoxic metabolite amended with C:N of 10:1 showed a strong herbicidal, high mortality and a significant decrease in dry weight on the seedling on the tested weeds. This demonstrated hypothetically the optimization influence of carbon (sucrose)–nitrogen (peptone) manipulations either in variable combination or as single nutrient based on the cultural development of strain C1136. Spores, mycelium biomass and biocontrol efficacy of the fungus all experienced accretion in sucrose and peptone nutrient environment and likewise support their use as an attractive alternative in the optimization of mycoherbicidal assays.

Keywords: Mycelium biomass; Nitrogen; Carbon; Sporulation; C:N ratio; Mycoherbicide (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://link.springer.com/10.1007/s10668-018-0273-1 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:spr:endesu:v:22:y:2020:i:3:d:10.1007_s10668-018-0273-1

Ordering information: This journal article can be ordered from
http://www.springer.com/economics/journal/10668

DOI: 10.1007/s10668-018-0273-1

Access Statistics for this article

Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development is currently edited by Luc Hens

More articles in Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development from Springer
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().